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Utilization of Working Silver Solid Electrode for Voltammetric Determination of Drug Metronidazole
Mráček, Ondřej ; Vyskočil, Vlastimil (advisor) ; Fischer, Jan (referee)
This Bachelor thesis discusses the electrochemical behavior of metronidazole and seeks to find optimal conditions for the drug's voltammetric determination at a working silver solid electrode (AgSE) using differential pulse voltammetry (DPV). The voltammetric behavior of the drug was investigated, where the dependent variable was the pH of a water medium Britton-Robinson buffer (BRB). The optimum medium for voltammetric determination of metronidazole at a AgSE is a region of cathodic potential chosen to be the Britton-Robinson buffer, where the sodium hydroxide alkaline component (Na-BRB) has a pH of 10.0, and then measured further at voltammetric cell (volume of 10 ml) at concentration dependencies in the range of (1-1000)×10−6 mol/l. The limit of quantification (LOQ) and the limit of detection (LOD) were found to be 1.8×10−6 mol/l and 5.5×10−7 mol/l, respectively. In addition, the metronidazole was studied for the effect of electrochemical regeneration at the AgSE, where the parameters were: Nr = 150, Ein_r = 0 mV, Tin_r = 0.1 s, Efin_r = −1500 mV, Tfin_r = 0.1 s. The next section studies the electrochemical activity of the AgSE, which is not entirely ideal. The newly developed method might be applicable in the future for determining metronidazole through differential pulse voltammetry (DPV) to...
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